Ammunition detection using high frequency multibeam snippet backscatter information

Highlights • Improved position accuracy of up to 0.08 cm using SAPOS®-corrected DGNSS • High-resolution snippet backscatter to detect and monitor UXO and its environment • Combined evaluation of bathymetry, backscatter intensities, and sediments Abstract The present study reports the evaluation of snippet backscatter information gathered with a high-frequency multibeam echosounder system (200–400 kHz) due to their usability to detect ammunition of different sizes in shallow coastal waters. Besides the feasibility of the snippet backscatter data, it was focused on the attainable horizontal accuracy in comparison to side-scan sonar and autonomous underwater vehicle (AUV) surveys. The data was collected in shallow coastal waters of up to 18 m water depth (Baltic Sea) close to an ammunition dumping site characterized by an almost flat seafloor covered with sand and silt sediments. The analysis of the multibeam compared to sidescan data indicates the snippet backscatter to be a promising prospective method for ammunition detection and being able to improve horizontal position accuracy of up to 0.08 m

Sediments and bedforms of the Harle tidal inlet (Wadden Sea, Germany)

ABSTRACTThe paper presents a map of sediment surface distribution and bedforms in the Harle tidal inlet, German Wadden Sea. Data collection, processing, and map editing were realized within the sublittoral mapping program of Lower Saxony national waters carried out by the NLWKN – Coastal Research Station. The map is the result of the combined use of multibeam echosounder, sub-bottom profiler, and ground-truth data. The sediment characterization is achieved by an unsupervised approach using an Object-Based Image Analysis (OBIA) on a normalized backscatter mosaic, verified by nineteen sediment samples. Morphometrical parameters and sub-bottom data provided important information to identify hard substrates and bedforms

Feasibility of Objective Seabed Mapping Techniques in a Coastal Tidal Environment (Wadden Sea, Germany)

The growing interest in monitoring the marine environment has strongly encouraged governmental agencies and research institutes to undertake seabed mapping programs and stimulated scientific interest in innovative mapping methods and tools. In this study, object-based image analysis was used to map a very shallow tidal inlet, characterized by high sediment variability and intense morphodynamic processes. The aim was to test the feasibility of reproducible mapping approaches within extended mapping programs of complex coastal areas. The study is based on full-coverage, high-resolution bathymetry and reflectivity, calibrated by means of sediment samples. Seafloor segmentation and classification were based on a cluster analysis performed on reflectivity, slope, and ruggedness. Statistics of clusters were extracted and analysed to identify the optimal number of clusters and evaluate the suitability of the clustering process to differentiate different seabed types. Clusters and samples data were joined to create a training and validation dataset for characterizing the seabed and carrying out an accuracy assessment. Misclassifications were explored and referred to three main reasons: (i) The not-perfect correspondence between sediment boundaries of classification systems and boundaries derived from the clustering process; (ii) the geomorphological features of the seabed; and (iii) the position accuracy of samples. The study contributes to testing of the feasibility of objective methods and highlights the importance of joining acoustic, lithological, and geomorphological analysis. It highlights issues and the need to critically analyse the mapping results and improve the accuracy of collected data

Common Pathological Mutations in PQBP1 Induce Nonsense-Mediated mRNA Decay and Enhance Exclusion of the Mutant Exon

The polyglutamine binding protein 1 (PQBP1) gene plays an important role in X-linked mental retardation (XLMR). Nine of the thirteen PQBP1 mutations known to date affect the AG hexamer in exon 4 and cause frameshifts introducing premature termination codons (PTCs). However, the phenotype in this group of patients is variable. To investigate the pathology of these PQBP1 mutations, we evaluated their consequences on mRNA and protein expression. RT-PCRs revealed mutation-specific reduction of PQBP1 mRNAs carrying the PTCs that can be partially restored by blocking translation, thus indicating a role for the nonsense-mediated mRNA decay pathway. In addition, these mutations resulted in altered levels of PQBP1 transcripts that skipped exon 4, probably as a result of altering important splicing motifs via nonsense-associated altered splicing (NAS). This hypothesis is supported by transfection experiments using wild-type and mutant PQBP1 minigenes. Moreover, we show that a truncated PQBP1 protein is indeed present in the patients. Remarkably, patients with insertion/deletion mutations in the AG hexamer express significantly increased levels of a PQBP1 isoform, which is very likely encoded by the transcripts without exon 4, confirming the findings at the mRNA level. Our study provides significant insight into the early events contributing to the pathogenesis of the PQBP1 related XLMR disease. Hum Mutat 31:90–98, 2010